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To increment the usage counter for AI Hacking, you’ll actually have to hack a synthetic enemy.

Use the AI Hacking ability 25 times to unlock this achievement. Engineers and Tali have access to this ability. Unlike the other abilities, you must actually take control of a hostile enemy to increment the usage counter on this ability, and since mid-to-high tier enemies are immune to the effects of the base ability, you’ll have to invest considerably more time (to level up) and talent points to effectively grind this achievement as compared to the other ability usage achievements. Level it up as much as possible and hack every turret, drone and geth you encounter until it unlocks.